Hygiene of populated placesISSN: 2707-0441 eISSN: 2707-045X
Issue 71, 2021   -   Pages: 117-125
Z.. Oliinyk1, O.V. Surmasheva1, Yu.V. Zheludenko1

: 613.49:579.63

Objective. Conduct microbiological testing of toothpaste samples with verification of tech-niques to confirm the ability to detect microorganisms in the presence of the toothpaste.
Materials and methods. Test samples of toothpaste with active carbon content were tested according to GOST 7983-99. A number of mediums, test microorganisms and principles of methodological verification were used in accordance with State Pharmacopoeia of Ukraine.
Results. The microorganisms growth in the negative controls was not observed, in all positive controls a typical growth of used museum test strains was observed - study results were valid. Obtained results of samples microbiology testing satisfy the State Sanitary Rules and Regulations requirements. According to the methods verification results it was found that the methods used to determine P. aeruginosa and Enterobacteriaceae were qualified while QMAFAnM, S. aureus, yeast and molds determination methods were considered unsuitable (the microorganisms test strains growth in the toothpaste sample presence was absent, indicating the antimicrobial activity). It has been proved that transfer to solid medium should be done regardless of the growth signs in the enrichment medium. The toothpastes antimicrobial activity may be lead to false-negative results of microbiology testing.
Conclusions. Studies have shown the microbiological parameters determining methods imperfection in the interstate standard GOST 7983-99 Toothpastes. General technical specifications. It is recommended to further perform toothpaste samples microbiology testing using the antimicrobial action neutralization method verified for each sample and invariably reseeding from enrichment medium to solid medium.

toothpaste, microbial attributes, methods, method verification.

1. DSaNPiN Derzhavni sanitarni pravyla i normy bezpeky produktsii parfumerno-kosmetychnoi promyslovosti [SSRSS State Sanitary Rules and Safety Standards for Products of the Perfume and Cosmetics Industry] (in Ukrainian).
2. Gosudarstvennaya farmakopeya SSSR. Vypusk 2. Metody mikrobiologicheskogo kontrolya lekarstvennykh sredstv [State Pharmacopoeia of the USSR. Issue 2. Methods for Microbiological Control of Medicines]. Moscow. 1990 ; 2 : 87-200 (in Russian).
3. Derzhavna Farmakopeia Ukrainy, 2 vydannia [State Pharmacopoeia of Ukraine, 2nd Edition]. Kharkiv. 2015 (in Ukrainian).
4. DSTU 8446:2015 Produkty kharchovi. Metody vyznachennia kilkosti mezofilnykh aerobnykh ta fakultatyvno-anaerobnykh mikroorhanizmiv [SSTU 8446:2015 Food Products. Methods for De-termining the Number of Mesophilic Aerobic and Facultative Anaerobic Microorganisms] (in Ukrainian).
5. DSTU ISO 16212:2018 (ISO 16212:2017, IDT) Kosmetyka. Mikrobiolohiia. Pidrakhunok kil-kosti drizhdzhiv ta plisniavy [SSTU ISO 16212:2018 (ISO 16212:2017, IDT) Cosmetics. Mi-crobiology. Counting the Amount of Yeast and Mold] (in Ukrainian).
6. DSTU 3034-95 Shampuni ta pinomyini zasoby. Mikrobne zabrudnennia. Metod vyiavlennia bakterii Escherichia coli [DSTU 3034-95 Shampoos and Foaming Agents. Microbial Contami-nation. Method for Detecting Escherichia Coli Bacteria] (in Ukrainian).
7. Surmasheva O.V., Oliinyk Z.A., Romanenko L.I., Mikhiienkova A.I., Nikonova N.O. Aktualni problemy mikrobiolohichnoho kontroliu yakosti parfumerno-kosmetychnoi produktsii, hihiienichno-profilaktychnykh zasobiv ta predmetiv osobystoi hihiieny [Actual Problems of Mi-crobiological Quality Control of Perfumery and Cosmetics, Hygienic and Preventive Means and Personal Hygiene ITEMS]. In : Hihiiena naselenykh mists [Hygiene of Populated Places]. 2017 ; 67 : 93-98 (in Ukrainian). DOI : https://doi.org/10.32402/hygiene2017.67.093
8. GOST 7983-99 Pasty zubnye. Obshchie tekhnicheskie usloviya [GOST 7983-99 Toothpastes. General Technical Conditions] (in Russian).
9. DSTU EN ISO/IEC 17025:2019 Zahalni vymohy do kompetentnosti vyprobuvalnykh ta kalibruvalnykh laboratorii (EN ISO/IEC 17025:2017, IDT; ISO/IEC 17025:2017, IDT) [SSTU EN ISO/IEC 17025:2019 General Requirements for the Competence of Testing and Calibration Laboratories (EN ISO/IEC 17025:2017, IDT; ISO/IEC 17025:2017, IDT)] (in Ukrainian).
10. DSTU ISO 21149:2010 (ISO 21149:2006, IDT) Zasoby kosmetychni. Mikrobiolohiia. Perelik ta vyiavliannia mezofilnykh aerobnykh bakterii [SSTU ISO 21149:2010 (ISO 21149:2006, IDT) Cosmetics. Microbiology. List and Detection of Mesophilic Aerobic Bacteria] (in Ukrainian).
11. DSTU ISO 21150:2010 (ISO 21150:2006, IDT) Zasoby kosmetychni. Mikrobiolohiia. Vyiavlennia Escherihia coli [SSTU ISO 21150:2010 (ISO 21150:2006, IDT) Cosmetics. Microbiol-ogy. Detection of Escherihia Coli] (in Ukrainian).
12. DSTU ISO 22717:2010 (ISO 22717:2006, IDT) Zasoby kosmetychni. Mikrobiolohiia. Metod vyiavliannia Pseudomonas aeruginosa [SSTU ISO 22717:2010 (ISO 22717:2006, IDT) Cosmetics. Microbiology. Method of Detecting Pseudomonas Aeruginosa] (in Ukrainian).
13. DSTU ISO 22718:2010 (ISO 22718:2006, IDT) Zasoby kosmetychni. Mikrobiolohiia. Metod vyiavliannia Staphylococcus aureus [SSTU ISO 22718:2010 (ISO 22718:2006, IDT) Cosmetics. Microbiology. Method of Detecting Staphylococcus Aureus] (in Ukrainian).
14. DSTU ISO 18416:2017 (ISO 18416:2015, IDT) Zasoby kosmetychni. Mikrobiolohiia. Metod vyiavliannia. Candida albicans [SSTU ISO 18416:2017 (ISO 18416:2015, IDT) Cosmetics. Microbiology. Detection Method. Candida Albicans] (in Ukrainian).
15. DSTU EN ISO 17516:2016 (EN ISO 17516:2014, IDT) Kosmetika. Mikrobiologiya. Mikrobiologicheskie predely [SSTU EN ISO 17516:2016 (EN ISO 17516:2014, IDT) Cosmetics. Microbiology. Microbiological Limits] (in Russian).

Oliinyk Z.., Surmasheva O.V., Zheludenko Yu.V. Metody kontroliu yakosti zubnykh past za mikrobiolohichnymy pokaznykamy v Ukraini [Microbiological Methods for Toothpastes Quality Control in Ukraine]. In : Hihiiena naselenykh mists : zb. nauk. pr. [Hygiene of Populated Places]. Kyiv ; 2021 ; 71 : 117-125 (in Ukrainian).